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human llc1  (ATCC)


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    ATCC human llc1
    Human Llc1, supplied by ATCC, used in various techniques. Bioz Stars score: 96/100, based on 282 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mouse+lung+carcinoma+ll+2+llc1/LL%2F2+(LLC1)%3B+Lewis+Lung+Carcinoma%3B+Mouse/pmc12990376-63-0-3
    Average 96 stars, based on 282 article reviews
    human llc1 - by Bioz Stars, 2026-09
    96/100 stars

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    Cell Culture:

    Article Title: Targeting PAR-2 with a negative allosteric modulator increases tumor antigen presentation and potentiates anti-PD-1 immunotherapy
    Article Snippet: 4T1.2 and B16F10-OVA were cultured in RPMI 1640 (cat. 350-015 CL, Wisent) containing 10% FBS (Gibco). .. HEK293 (RRID: CVCL_0045) and mouse lung carcinoma LL/2 (LLC1) (RRID: CVCL_4358) cell lines were obtained from the American Type Culture Collection and cultured in DMEM (cat. 61965-026, Gibco, for HEK293 and cat. 41966-029, Gibco, for LLC1) supplemented with 10% fetal calf serum and 1% PS (100 U/mL penicillin and 100 μg/mL streptomycin; cat. 15140122, Wisent). ..

    Article Title: Targeting PAR-2 with a negative allosteric modulator increases tumor antigen presentation and potentiates anti-PD-1 immunotherapy.
    Article Snippet: 4T1.2 and B16F10- OVA were cultured in RPMI 1640 (cat. 350- 015 CL, Wisent) containing 10% FBS (Gibco). .. HEK293 (RRID: CVCL_0045) and mouse lung carcinoma LL/2 (LLC1) (RRID: CVCL_4358) cell lines were obtained from the American Type Culture Collection and cultured in DMEM (cat. 61965- 026, Gibco, for HEK293 and cat. 41966- 029, Gibco, for LLC1) supplemented with 10% fetal calf serum and 1% PS (100 U/mL penicillin and 100 μg/mL streptomycin; cat. 15140122, Wisent). ..



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    human llc1  (ATCC)
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    ll2  (ATCC)
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    High-resolution profiling of TIICs across murine syngeneic tumor models. (A) Schematic representation of the experimental workflow for isolating and processing Cd45+ immune cells from 10 murine tumor models: breast mammary carcinoma (4T1, EMT6, MMTV-PyMT), colon carcinoma (CT26.WT, MC38), glioma (GL261), renal adenocarcinoma (Renca), lung carcinoma <t>(LL2),</t> melanoma (B16F10), and pancreatic adenocarcinoma (Pan02), with three biological replicates per model. (B) UMAP visualization of CCA integrated data depicting 7 major immune cell populations. (C) Expression patterns of canonical marker genes utilized to identify lymphoid (T cells, NK cells, B cells) and myeloid (DCs, Mo/Mφs, neutrophils, mast cells) lineages. (D) Percentages of major immune cell types in TIICs for each model.
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    murine lewis lung carcinoma  (ATCC)
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    ATCC murine lewis lung carcinoma
    Effect of hypoxia on cell viability and metabolic reprogramming. ( A ) Cell viability of <t>murine</t> J774 and LLC cells and human A549 <t>lung</t> <t>carcinoma</t> cells cultured under normoxia or hypoxia for 24 and 48 h, showing no significant reduction in viability under hypoxic conditions, unpaired t -test. ( B ) Metabolic adaptations induced by hypoxia. Mitochondrial activity at 24 and 48h in LLC cultured under normoxia or hypoxia ( left ). Relative CAIX mRNA expression was significantly upregulated in LLC ( middle ). Hypoxic exposure also increased lactate concentration in the culture supernatant and decreased extracellular pH compared with normoxia ( right ). ( C ) Similar results were obtained in A549 cells. Data are shown as mean ± SEM of two independent experiments * p < 0.05, ** p < 0.01 and *** p < 0.001 versus normoxia; two-way ANOVA with Tukey’s multiple-comparison test.
    Murine Lewis Lung Carcinoma, supplied by ATCC, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    llc  (ATCC)
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    ATCC llc
    Effect of hypoxia on cell viability and metabolic reprogramming. ( A ) Cell viability of <t>murine</t> J774 and LLC cells and human A549 <t>lung</t> <t>carcinoma</t> cells cultured under normoxia or hypoxia for 24 and 48 h, showing no significant reduction in viability under hypoxic conditions, unpaired t -test. ( B ) Metabolic adaptations induced by hypoxia. Mitochondrial activity at 24 and 48h in LLC cultured under normoxia or hypoxia ( left ). Relative CAIX mRNA expression was significantly upregulated in LLC ( middle ). Hypoxic exposure also increased lactate concentration in the culture supernatant and decreased extracellular pH compared with normoxia ( right ). ( C ) Similar results were obtained in A549 cells. Data are shown as mean ± SEM of two independent experiments * p < 0.05, ** p < 0.01 and *** p < 0.001 versus normoxia; two-way ANOVA with Tukey’s multiple-comparison test.
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    atcc crl 1642 gift  (ATCC)
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    ATCC atcc crl 1642 gift
    Effect of hypoxia on cell viability and metabolic reprogramming. ( A ) Cell viability of <t>murine</t> J774 and LLC cells and human A549 <t>lung</t> <t>carcinoma</t> cells cultured under normoxia or hypoxia for 24 and 48 h, showing no significant reduction in viability under hypoxic conditions, unpaired t -test. ( B ) Metabolic adaptations induced by hypoxia. Mitochondrial activity at 24 and 48h in LLC cultured under normoxia or hypoxia ( left ). Relative CAIX mRNA expression was significantly upregulated in LLC ( middle ). Hypoxic exposure also increased lactate concentration in the culture supernatant and decreased extracellular pH compared with normoxia ( right ). ( C ) Similar results were obtained in A549 cells. Data are shown as mean ± SEM of two independent experiments * p < 0.05, ** p < 0.01 and *** p < 0.001 versus normoxia; two-way ANOVA with Tukey’s multiple-comparison test.
    Atcc Crl 1642 Gift, supplied by ATCC, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mouse+lung+carcinoma+ll+2+llc1/LL%2F2+(LLC1)%3B+Lewis+Lung+Carcinoma%3B+Mouse/pmc12513485__jciinsight-10-192981-s102-92-213-213
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    Image Search Results


    High-resolution profiling of TIICs across murine syngeneic tumor models. (A) Schematic representation of the experimental workflow for isolating and processing Cd45+ immune cells from 10 murine tumor models: breast mammary carcinoma (4T1, EMT6, MMTV-PyMT), colon carcinoma (CT26.WT, MC38), glioma (GL261), renal adenocarcinoma (Renca), lung carcinoma (LL2), melanoma (B16F10), and pancreatic adenocarcinoma (Pan02), with three biological replicates per model. (B) UMAP visualization of CCA integrated data depicting 7 major immune cell populations. (C) Expression patterns of canonical marker genes utilized to identify lymphoid (T cells, NK cells, B cells) and myeloid (DCs, Mo/Mφs, neutrophils, mast cells) lineages. (D) Percentages of major immune cell types in TIICs for each model.

    Journal: Frontiers in Immunology

    Article Title: Single-cell atlas of the tumor immune microenvironment across syngeneic murine models

    doi: 10.3389/fimmu.2025.1676581

    Figure Lengend Snippet: High-resolution profiling of TIICs across murine syngeneic tumor models. (A) Schematic representation of the experimental workflow for isolating and processing Cd45+ immune cells from 10 murine tumor models: breast mammary carcinoma (4T1, EMT6, MMTV-PyMT), colon carcinoma (CT26.WT, MC38), glioma (GL261), renal adenocarcinoma (Renca), lung carcinoma (LL2), melanoma (B16F10), and pancreatic adenocarcinoma (Pan02), with three biological replicates per model. (B) UMAP visualization of CCA integrated data depicting 7 major immune cell populations. (C) Expression patterns of canonical marker genes utilized to identify lymphoid (T cells, NK cells, B cells) and myeloid (DCs, Mo/Mφs, neutrophils, mast cells) lineages. (D) Percentages of major immune cell types in TIICs for each model.

    Article Snippet: LL2 , ATCC (CRL-1642) , , DMEM (Gibco, Cat# 11965092) supplemented with 10% (v/v) fetal bovine serum (Gibco, Cat# 10099-141) and 100 ug/ml of penicillin and streptomycin (Gibco, Cat# 15140-122).

    Techniques: Expressing, Marker

    Effect of hypoxia on cell viability and metabolic reprogramming. ( A ) Cell viability of murine J774 and LLC cells and human A549 lung carcinoma cells cultured under normoxia or hypoxia for 24 and 48 h, showing no significant reduction in viability under hypoxic conditions, unpaired t -test. ( B ) Metabolic adaptations induced by hypoxia. Mitochondrial activity at 24 and 48h in LLC cultured under normoxia or hypoxia ( left ). Relative CAIX mRNA expression was significantly upregulated in LLC ( middle ). Hypoxic exposure also increased lactate concentration in the culture supernatant and decreased extracellular pH compared with normoxia ( right ). ( C ) Similar results were obtained in A549 cells. Data are shown as mean ± SEM of two independent experiments * p < 0.05, ** p < 0.01 and *** p < 0.001 versus normoxia; two-way ANOVA with Tukey’s multiple-comparison test.

    Journal: International Journal of Molecular Sciences

    Article Title: 4-Methylumbelliferone Modulates CAIX to Mitigate Hypoxia-Driven Dysregulation and Enhance PD-1 Immunotherapy in Lung Cancer

    doi: 10.3390/ijms262110427

    Figure Lengend Snippet: Effect of hypoxia on cell viability and metabolic reprogramming. ( A ) Cell viability of murine J774 and LLC cells and human A549 lung carcinoma cells cultured under normoxia or hypoxia for 24 and 48 h, showing no significant reduction in viability under hypoxic conditions, unpaired t -test. ( B ) Metabolic adaptations induced by hypoxia. Mitochondrial activity at 24 and 48h in LLC cultured under normoxia or hypoxia ( left ). Relative CAIX mRNA expression was significantly upregulated in LLC ( middle ). Hypoxic exposure also increased lactate concentration in the culture supernatant and decreased extracellular pH compared with normoxia ( right ). ( C ) Similar results were obtained in A549 cells. Data are shown as mean ± SEM of two independent experiments * p < 0.05, ** p < 0.01 and *** p < 0.001 versus normoxia; two-way ANOVA with Tukey’s multiple-comparison test.

    Article Snippet: Murine Lewis lung carcinoma (LLC, ATCC CRL-1642 kindly transferred by Dra.

    Techniques: Cell Culture, Activity Assay, Expressing, Concentration Assay, Comparison